Sr26 is effective against all stem rust races in Australia and worldwide
Key Information
Crop
Wheat
Disease resistance(s)
Wheat stem rust
Resistance type
All stage resistance
Source
Agropyron elongatum
First reported
Knott DR (1961) The inheritance of rust resistance. VI. The transfer of stem rust resistance from Agropyron elongatum to common wheat. Can J Plant Sci 41:109–123
Chromosomal location
6A
Genomic Sequence
MN531843
Germplasm availability
Already present in Australian wheats e.g., LRPB Hellfire, LRPB Spitfire, SEA Condamine
Additional Information
A combination (i.e. multiplexing) of two dominant PCR markers Sr26#43 and BE18379 in repulsion phase provides a diagnostic co-dominant marker for Sr26
Markers
PCR
| Marker Type | Marker Name | Forward Primer | Reverse Primer | Annealing temperature (°C) | Size (bp): Resistant allele/Susceptible allele | Published location |
|---|---|---|---|---|---|---|
| PCR | Sr26GS | GGAATACTCGAATACCAGGCCAT | TTGCCACTGTGAACATGTTTATAGAT | 58 | Zhang, J., Hewitt, T.C., Boshoff, W.H.P. et al. A recombined Sr26 and Sr61 disease resistance gene stack in wheat encodes unrelated NLR genes. Nat Commun 12, 3378 (2021). https://doi.org/10.1038/s41467-021-23738-0 | |
| PCR | Sr26#43 | AATCGTCCACATTGGCTTCT | CGCAACAAAATCATGCACTA | 60 | 207bp | Mago, R., Bariana, H.S., Dundas, I.S. et al. Development of PCR markers for the selection of wheat stem rust resistance genes Sr24 and Sr26 in diverse wheat germplasm. Theor Appl Genet 111, 496–504 (2005). https://doi.org/10.1007/s00122-005-2039-z |
| PCR | BE518379 | AGCCGCGAAATCTACTTTGA | TTAAACGGACAGAGCACACG | 60 | 303bp | https://maswheat.ucdavis.edu/protocols/Sr26 |